Chromatographic separation system is achieved by a column with the stationary phase filling. The chromatographic separation system adopts the automatic simulated moving bed technology, which can realize the separation of two and multiple components efficiently. Equipment operation is not only low consumption, high production capacity, but also can make the product purity, safe and stable operation.The automated simulated moving bed chromatography separation system can be used for the separation of fructose and glucose, the separation of fructose and psicose, the purification of tryptophan and the recovery of glycine mother liquor. In addition, this system can also be used in the production of starch sugars, organic acids, and in the production of antibiotics.